Tylenol & Liver Health: Glutathione Boost Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. The Use and Potential Benefits of N Acetylcysteine in Non Acetaminophen Acute Liver Failure: An Etiology Based Review Downregulation of Glutathione Mediated Detoxification Capacity by Binge Drinking Aggravates Acetaminophen Induced Liver Injury through IRE1 ER Stress Signaling